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93
Hycult Biotech human complement properdin elisa kit
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Human Complement Properdin Elisa Kit, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alexion Phrama anti properdin monoclonal antibody 14e1
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Anti Properdin Monoclonal Antibody 14e1, supplied by Alexion Phrama, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quidel human properdin protein
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Human Properdin Protein, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quidel human purified properdin
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Human Purified Properdin, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Quidel human properdin antigen
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Human Properdin Antigen, supplied by Quidel, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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93
Hycult Biotech elisa kits
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Elisa Kits, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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NovelMed Therapeutics nm9401 properdin
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Nm9401 Properdin, supplied by NovelMed Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems rhproperdin
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Rhproperdin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
NovelMed Therapeutics anti-properdin
(A) Measurement by <t>ELISA</t> of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Anti Properdin, supplied by NovelMed Therapeutics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Measurement by ELISA of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).

Journal: Frontiers in Immunology

Article Title: Expression of Properdin, the positive regulator of the Complement Alternative Pathway, at the fetal-maternal interface in Preeclampsia

doi: 10.3389/fimmu.2025.1739327

Figure Lengend Snippet: (A) Measurement by ELISA of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).

Article Snippet: For measuring the levels of properdin in PE and CTRL sera, human complement properdin ELISA kit (Hycult Biotech; # HK334) was used; instructions provided by the manufacturer were followed for the assay.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, MANN-WHITNEY