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Journal: Frontiers in Immunology
Article Title: Expression of Properdin, the positive regulator of the Complement Alternative Pathway, at the fetal-maternal interface in Preeclampsia
doi: 10.3389/fimmu.2025.1739327
Figure Lengend Snippet: (A) Measurement by ELISA of circulating properdin levels in sera of CTRL and PE women, collected during 3 rd trimester. Lower levels of properdin were observed in PE pregnancies compared to CTRL, showing a highly significant difference ( n = 20 PE vs n = 20 CTRL). ** p < 0.01 (paired Wilcoxon signed-rank test). (B) TEM image of: i) PE placenta showing syncytiotrophoblast fragments as extracellular vesicles of varied sizes, cell debris, apoptotic nuclei, possibly released towards the intervillous space. The exosomes are marked with yellow arrowheads, STBMs with green arrowheads, and pyknotic nuclei with red arrowheads (scale 500 nm). ii) CTRL placenta showing almost an intact syncytiotrophoblast layer (outer layer facing intervillous space) and cytotrophoblast layer lying underneath. (C) Evaluation of properdin deposition on placental STBMs via Western blot (WB). STBMs derived from PE ( n = 3) and CTRL ( n = 3) were probed with anti-properdin antibody. β-actin was used to normalize the results. (D) Evaluation of properdin levels in circulating placental exosomes of 3 rd trimester via WB. Properdin level was significantly decreased in PE-derived placental exosomes compared to CTRL. Proteins extracted from circulating placental exosomes from PE ( n = 4) and CTRL ( n = 4) women were probed with mouse anti-human properdin primary antibody. The WB data is represented as mean ± SEM. CD9 was used to normalize the results. * p < 0.05 (Mann-Whitney U test).
Article Snippet: For measuring the levels of properdin in PE and CTRL sera,
Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Derivative Assay, MANN-WHITNEY